2024-03-29T02:36:51Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1616312021-06-30T07:49:20Zcom_10261_34com_10261_5com_10261_70com_10261_2col_10261_287col_10261_323
00925njm 22002777a 4500
dc
Tomás-Cortázar, Julen
author
Plaza-Vinuesa, Laura
author
Rivas, Blanca de las
author
Lavín, José Luis
author
Barriales, Diego
author
Abecia, Leticia
author
Mancheño, Jose M.
author
Aransay, Ana M.
author
Muñoz, Rosario
author
Anguita, Juan
author
Rodríguez, Héctor
author
2018-02-26
[Background]: Tannases are tannin-degrading enzymes that have been described in fungi and bacteria as an adaptative mechanism to overcome the stress conditions associated with the presence of these phenolic compounds. [Results]: We have identified and expressed in E. coli a tannase from the oral microbiota member Fusobacterium nucleatum subs. polymorphum (TanBFnp). TanBFnp is the first tannase identified in an oral pathogen. Sequence analyses revealed that it is closely related to other bacterial tannases. The enzyme exhibits biochemical properties that make it an interesting target for industrial use. TanBFnp has one of the highest specific activities of all bacterial tannases described to date and shows optimal biochemical properties such as a high thermal stability: the enzyme keeps 100% of its activity after prolonged incubations at different temperatures up to 45 °C. TanBFnp also shows a wide temperature range of activity, maintaining above 80% of its maximum activity between 22 and 55 °C. The use of a panel of 27 esters of phenolic acids demonstrated activity of TanBFnp only against esters of gallic and protocatechuic acid, including tannic acid, gallocatechin gallate and epigallocatechin gallate. Overall, TanBFnp possesses biochemical properties that make the enzyme potentially useful in biotechnological applications.
[Conclusions]: We have identified and characterized a metabolic enzyme from the oral pathogen Fusobacterium nucleatum subsp. polymorphum. The biochemical properties of TanBFnp suggest that it has a major role in the breakdown of complex food tannins during oral processing. Our results also provide some clues regarding its possible participation on bacterial survival in the oral cavity. Furthermore, the characteristics of this enzyme make it of potential interest for industrial use.
Microbial Cell Factories 17(1): 33 (2018)
1475-2859
http://hdl.handle.net/10261/161631
10.1186/s12934-018-0880-4
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100003086
29482557
Identification of a highly active tannase enzyme from the oral pathogen Fusobacterium nucleatum subsp. polymorphum