2024-03-28T23:09:04Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1535232021-12-27T15:32:44Zcom_10261_11773com_10261_1col_10261_11774
00925njm 22002777a 4500
dc
López-Contreras, Andrés J.
author
Rojas, A. M.
author
Fernández-Capetillo, Óscar
author
2013-04-25
DNA replication is facilitated by multiple factors that concentrate in the vicinity of replication forks. Here, we developed an approach that combines the isolation of proteins on nascent DNA chains with mass spectrometry (iPOND-MS), allowing a comprehensive proteomic characterization of the human replisome and replisome-associated factors. In addition to known replisome components, we provide a broad list of proteins that reside in the vicinity of the replisome, some of which were not previously associated with replication. For instance, our data support a link between DNA replication and the Williams-Beuren syndrome and identify ZNF24 as a replication factor. In addition, we reveal that SUMOylation is widespread for factors that concentrate near replisomes, which contrasts with lower UQylation levels at these sites. This resource provides a panoramic view of the proteins that concentrate in the surroundings of the replisome, which should facilitate future investigations on DNA replication and genome maintenance.
Cell Reports 3(4): 1105-1116 (2013)
2211-1247
http://hdl.handle.net/10261/153523
10.1016/j.celrep.2013.03.009
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/100004435
http://dx.doi.org/10.13039/100000011
http://dx.doi.org/10.13039/501100000781
23545495
A Proteomic Characterization of Factors Enriched at Nascent DNA Molecules