2024-03-28T18:00:46Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1246582021-11-22T12:53:05Zcom_10261_41com_10261_1col_10261_294
00925njm 22002777a 4500
dc
Reid, Juliet L.
author
Bannister, Andrew J.
author
Zegerman, Philip
author
Martínez-Balbás, Marian
author
Kouzarides, Tony
author
1998-08-03
The P/CAF protein has intrinsic histone acetyltransferase (HAT) activity and is capable of binding the transcriptional co-activator CBP. Here we show that P/CAF can regulate transcription and that this function is independent of its binding to CBP. The HAT domain of P/CAF has transcriptional activation potential in yeast. In mammalian cells P/CAF can stimulate transcription of the RSV promoter, using the activity of its HAT domain. We show that the adenovirus protein E1A targets P/CAF and sequesters its transcriptional activity. Binding of E1A to P/CAF is direct, independent of CBP and requires residues within E1A conserved region 1. We find that the P/CAF binding residues in E1A are within a motif shown to be essential for efficient disruption of myogenesis by E1A. The fact that E1A can directly bind and regulate the activity of P/CAF, independently of its regulation of CBP, highlights an important role for P/CAF in the process of cell differentiation
EMBO Journal 17(15): 4469-4477 (1998)
0261-4189
http://hdl.handle.net/10261/124658
10.1093/emboj/17.15.4469
http://dx.doi.org/10.13039/501100000265
http://dx.doi.org/10.13039/501100003043
9687513
Acetyltransferase
CAF
E1A
Myogenesis
P
Transcription
E1A directly binds and regulates the P/CAF acetyltransferase