2024-03-28T08:15:14Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1148952021-12-28T16:18:24Zcom_10261_70com_10261_2col_10261_323
00925njm 22002777a 4500
dc
Esteban-Torres, María
author
Landete, José María
author
Reverón, Inés
author
Santamaría, Laura
author
Rivas, Blanca de las
author
Muñoz, Rosario
author
2015
© 2015, American Society for Microbiology. Lactobacillus plantarum is the lactic acid bacterial species most frequently found in the fermentation of food products of plant origin on which phenolic compounds are abundant. L. plantarum strains showed great flexibility in their ability to adapt to different environments and growth substrates. Of 28 L. plantarum strains analyzed, only cultures from 7 strains were able to hydrolyze hydroxycinnamic esters, such as methyl ferulate or methyl caffeate. As revealed by PCR, only these seven strains possessed the est_1092 gene. When the est_1092 gene was introduced into L. plantarum WCFS1 or L. lactis MG1363, their cultures acquired the ability to degrade hydroxycinnamic esters. These results support the suggestion that Est_1092 is the enzyme responsible for the degradation of hydroxycinnamic esters on the L. plantarum strains analyzed. The Est_1092 protein was recombinantly produced and biochemically characterized. Surprisingly, Est_1092 was able to hydrolyze not only hydroxycinnamic esters, since all the phenolic esters assayed were hydrolyzed. Quantitative PCR experiments revealed that the expression of est_1092 was induced in the presence of methyl ferulate, an hydroxycinnamic ester, but was inhibited on methyl gallate, an hydroxybenzoic ester. As Est_1092 is an enzyme active on a broad range of phenolic esters, simultaneously possessing feruloyl esterase and tannase activities, its presence on some L. plantarum strains provides them with additional advantages to survive and grow on plant environments.
Applied and Environmental Microbiology 81(9): 3235- 3242 (2015)
http://hdl.handle.net/10261/114895
10.1128/AEM.00323-15
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/100007652
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100003339
25746986
A Lactobacillus plantarum esterase active on a broad range of phenolic esters