2024-03-29T05:19:21Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1100342021-12-28T15:52:28Zcom_10261_41com_10261_1com_10261_25col_10261_294col_10261_278
00925njm 22002777a 4500
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Tabone, Mariangela
author
Lioy, Virginia S.
author
Ayora, Silvia
author
Machón, Cristina
author
Alonso, Juan Carlos
author
2014-01-29
A fraction of otherwise antimicrobial-sensitive Bacillus subtilis cells, called persisters, are phenotypically tolerant of antimicrobial treatment. We report that, independently of B. subtilis' growth phase, transient ζ toxin expression induces a dormant state and alters cellular responses so that cells are more sensitive to antimicrobials with different modes of action. This outcome is modulated by fine tuning (p)ppGpp and GTP levels: i) in the presence of low >dysregulated> (p)ppGpp levels (as in relA- cells) hyper-tolerance to both toxin and antimicrobials was observed; ii) physiological or low (p)ppGpp levels (as in the wild-type, sasA-, sasB- and relA- sasA- context) show a normal toxin and antimicrobial tolerance; and iii) lower levels (in relA- sasB -) or absence of (p)ppGpp (in the relA- sasA- sasB- context), in concert with elevated GTP levels, potentiate the efficacy of both toxin and antimicrobial action, rendering tolerance vulnerable to eradication. © 2014 Tabone et al.
PLoS ONE 9(1): e86615 (2014)
http://hdl.handle.net/10261/110034
10.1371/journal.pone.0086615
24489751
Cellular stress responses
Bacillus subtilis
Antitoxins
Toxins
Cell physiology
Confidence intervals
Gene pool
Antimicrobials
Role of toxin ζ and starvation responses in the sensitivity to antimicrobials