2024-03-28T14:33:03Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/551462019-05-09T07:35:02Zcom_10261_68com_10261_2col_10261_321
2012-08-28T12:27:03Z
urn:hdl:10261/55146
Characterization of glycolytic initial metabolites and enzyme activities in developing sunflower (Helianthus annuus L.) seeds
Troncoso-Ponce, M. Adrián
Kruger, Nicholas J.
Ratcliffe, George
Garcés Mancheño, Rafael
Martínez-Force, Enrique
Unlike other oilseeds (e.g. Arabidopsis), developing sunflower seeds do not accumulate a lot of starch and they rely on the sucrose that comes from the mother plant to synthesise lipid precursors. Between 10 and 25 days after flowering (DAF), when sunflower seeds form and complete the main period of storage lipid synthesis, the sucrose content of seeds is relatively constant. By contrast, the glucose and fructose content falls from day 20 after flowering and it is always lower than that of sucrose, with glucose being the minor sugar at the end of the seed formation. By studying the apparent kinetic parameters and the activity of glycolytic enzymes in vitro, it is evident that all the components of the glycolytic pathway are present in the crude seed extract. However, in isolated plastids important enzymatic activities are missing, such as the glyceraldehyde-3-phosphate dehydrogenase, involved in the conversion of glyceraldehyde 3-phosphate into 1,3-biphospho-glycerate, or the enolase that converts 2-phosphoglycerate into phosphoenolpyruvate. Hence, phosphoenolpyruvate or one of its derivatives, like pyruvate and malate from the cytosol, may be the primary carbon sources for lipid biosynthesis. Accordingly, the glucose-6-P imported into the plastid is likely to be used in the pentose phosphate pathway to produce the reducing power for lipid biosynthesis in the form of NADPH. Data from crude seed extracts indicate that enolase activity increased during seed formation, from 16 days after flowering, and that this activity was well correlated with the period of storage lipid synthesis. In addition, while the presence of some glycolytic enzymes increased during lipid synthesis, others decreased, remained constant, or displayed irregular temporal behaviour.
2012-08-28T12:27:03Z
2012-08-28T12:27:03Z
2009-06
artículo
Phytochemistry 70(9): 1117-1122 (2009)
1873-3700
PMID: 19665153
http://hdl.handle.net/10261/55146
10.1016/j.phytochem.2009.07.012
eng
http://dx.doi.org/10.1016/j.phytochem.2009.07.012
closedAccess
Elsevier