2024-03-28T14:40:34Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/37402021-12-27T16:55:03Zcom_10261_64com_10261_1col_10261_317
2008-04-25T09:36:01Z
urn:hdl:10261/3740
The L1Tc non-LTR retrotransposon of Trypanosoma cruzi contains an internal RNA-pol II-dependent promoter that strongly activates gene transcription and generates unspliced transcripts
Heras, Sara R.
López López, Manuel Carlos
Olivares, Mónica
Thomas, María del Carmen
El copyright pertenece a Oxford University Press. La publicación original está disponible en http://nar.oxfordjournals.org/?code=nar&.cgifields=code
L1Tc is the best represented autonomous LINE of the Trypanosoma cruzi genome, throughout which several functional copies may exist. In this study, we show that the first 77 bp of L1Tc (Pr77) (also present in the T. cruzi non-autonomous retrotransposon NARTc, in the Trypanosoma brucei RIME/ingi elements, and in the T. cruzi, T. brucei and Leishmania major degenerate L1Tc/ingi-related elements [DIREs]) behave as a promoter element that activates gene transcription. The transcription rate promoted by Pr77 is 10–14-fold higher than that mediated by sequences located upstream from the T. cruzi tandemly repeated genes KMP11 and the GAPDH. The Pr77 promoter-derived mRNAs initiate at nucleotide +1 of L1Tc, are unspliced and translated. L1Tc transcripts show a moderate half life and are RNA pol II dependent. The presence of an internal promoter at the 5′ end of L1Tc favors the production of full-length L1Tc RNAs and reinforces the hypothesis that this mobile element may be naturally autonomous in its transposition.
2008-04-25T09:36:01Z
2008-04-25T09:36:01Z
2007-04
artículo
Nucleic Acids Res. 2007 April; 35(7): 2199–2214
0305-1048
http://hdl.handle.net/10261/3740
10.1093/nar/gkl1137
17369274
eng
openAccess
Oxford University Press