2024-03-28T08:32:49Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1956232022-09-21T09:43:15Zcom_10261_64com_10261_1col_10261_317
2019-11-26T12:44:33Z
urn:hdl:10261/195623
Changes in saliva of dogs with canine leishmaniosis: A proteomic approach
Franco-Martínez, Lorena
Tvarijonaviciute, Asta
Horvatić, Anita
Guillemin, Nicolas
Bernal, Luis J.
Barie Rafaj, Renata
Cerón, José J.
Thomas, María del Carmen
López López, Manuel Carlos
Tecles, Fernando
Martínez-Subiela, Silvia
Mrljak, Vladimir
Ministerio de Economía y Competitividad (España)
Fundación Séneca
European Commission
Universidad de Murcia
Franco-Martínez, Lorena [0000-0003-3375-4274]
Tvarijonaviciute, Asta [0000-0002-5323-5001]
Bernal, Luis J. [0000-0002-3648-7012]
Cerón, José J. [0000-0002-8654-1793]
Thomas, María del Carmen [0000-0003-3586-9657]
López López, Manuel Carlos [0000-0003-0002-3678]
Martínez-Subiela, Silvia [0000-0002-1524-1558]
Biomarkers
Saliva
Leishmaniosis
Dog
Gel-free proteomics
Tandem mass tag
In the present study, a quantitative proteomic approach to study changes in saliva proteins associated with canine leishmaniosis (CanL) was performed. For this, canine salivary proteins were analysed and compared between dogs before (TO) and after (T1) experimental infection with Leishmania infantum by high-throughput label-based quantitative LC-MS/MS proteomic approach and bioinformatic analysis of the in silico inferred interactome protein network was created from the initial list of differential proteins. More than 2000 proteins were identified, and of the 90 differentially expressed proteins between TO and T1, 12 were down-regulated with log2 fold change lower than -0.5849, and 19 were up-regulated with log2 fold change greater than 0.5849. This study provides evidence of changes in salivary proteome that can occur in canine leishmaniosis and revealed biological pathways in saliva modulated in canine leishmaniosis with potential for further targeted research.
2019-11-26T12:44:33Z
2019-11-26T12:44:33Z
2019-08
artículo
Veterinary Parasitology
0304-4017
http://hdl.handle.net/10261/195623
10.1016/j.vetpar.2019.06.014
1873-2550
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/100007801
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100004687
31395204
eng
Sí
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/RTC2016-50005-1
info:eu-repo/grantAgreement/EC/FP7/621394
closedAccess
Elsevier