2024-03-28T22:21:24Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1832932020-07-08T12:43:20Zcom_10261_101com_10261_5col_10261_354
2019-06-04T12:35:27Z
urn:hdl:10261/183293
Orientation and function of a membrane-bound enzyme monitored by electrochemical surface-enhanced infrared absorption spectroscopy
Gutiérrez-Sanz, Óscar
Marqués, Marta
Pereira, Inês A. C.
López de Lacey, Antonio
Lubitz, Wolfgang
Rüdiger Ortiz, Olaf
Ministerio de Economía y Competitividad (España)
SEIRA
Phospholipid bilayer membranes
Hydrogenase
Protein film electrochemistry
Electrochemical SEIRA is the method of choice for characterizing a lipid-bilayer-anchored, membrane-bound hydrogenase immobilized on a gold electrode. This setup allows the study of the enzyme under conditions mimicking its natural environment. A single experiment provides all of the crucial spectroscopic information relating to the protein orientation, active site of the protein, and the lipid bilayer and also direct electrochemical determination of the catalytic H2 oxidation by the enzyme.
2019-06-04T12:35:27Z
2019-06-04T12:35:27Z
2013-08-05
2019-06-04T12:35:27Z
artículo
Journal of Physical Chemistry Letters 4(17): 2794- 2798 (2013)
1948-7185
http://hdl.handle.net/10261/183293
10.1021/jz4013678
http://dx.doi.org/10.13039/501100003329
eng
https://doi.org/10.1021/jz4013678
Sí
closedAccess
American Chemical Society