2024-03-28T20:19:49Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1790722021-12-27T16:39:30Zcom_10261_38com_10261_5col_10261_291
2019-04-02T08:31:57Z
urn:hdl:10261/179072
Altered expression of the kisspeptin/KISS1R and neurokinin B/NK3R systems in mural granulosa and cumulus cells of patients with polycystic ovarian syndrome
Blasco, Víctor
Pinto Pérez, Francisco M.
Fernández- Atucha, Ainhoa
Prados, Nicolás
Tena-Sempere, Manuel
Fernández-Sánchez, Manuel
Candenas, M. Luz
Ministerio de Economía y Competitividad (España)
Cumulus cells
Granulosa cells
Kisspeptin
Neurokinin B
Polycystic ovarian syndrome
PURPOSE:
The neurokinin B (NKB)/NK3 receptor (NK3R) and kisspeptin (KISS1)/kisspeptin receptor (KISS1R), two systems essential for reproduction, are present in human granulosa cells (GCs) of healthy women and contribute to the control of fertility, at least partially, by acting on the gonads. However, little is known about the expression of these systems in GCs of women with polycystic ovarian syndrome (PCOS). The aim of this study was to analyze the expression of NKB/NK3R and KISS1/KISS1R in mural granulosa (MGCs) and cumulus cells (CCs) of PCOS women.
METHODS:
A cross-sectional study was performed in 46 healthy women and 43 PCOS women undergoing controlled ovarian stimulation. MGCs and CCs were collected from pre-ovulatory follicles after transvaginal ultrasound-guided oocyte retrieval and the expression of the genes encoding NKB (TAC3), NK3R (TACR3), KISS1, and its receptor (KISS1R) was analyzed using real-time quantitative RT-PCR.
RESULTS:
TAC3, TACR3, and KISS1 mRNA levels were decreased in MGCs and CCs of PCOS women. TAC3 positively correlated with KISS1 in MGCs of healthy women and TACR3 was positively associated with KISS1R in CCs from healthy women. These associations were not observed in PCOS women.
CONCLUSION:
The NKB/NK3R and KISS1/KISS1R systems are dysregulated in MGCs and CCs of PCOS women. The lower expression of these systems in GCs could contribute to the abnormal follicle development and defective ovulation that characterize the pathogenesis of PCOS.
2019-04-02T08:31:57Z
2019-04-02T08:31:57Z
2019
artículo
Journal of Assisted Reproduction and Genetics, 36(1): 113-120 (2019)
http://hdl.handle.net/10261/179072
10.1007/s10815-018-1338-7
http://dx.doi.org/10.13039/501100003329
30382469
eng
Postprint
https://doi.org/10.1007/s10815-018-1338-7
Sí
openAccess
Springer