2024-03-29T15:12:37Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1663232021-12-28T16:32:13Zcom_10261_79com_10261_1col_10261_332
2018-06-14T10:45:47Z
urn:hdl:10261/166323
Phenotyping and susceptibility of established porcine cells lines to African Swine Fever Virus infection and viral production
Sánchez, Elena G.
Riera, Elena
Nogal, Marisa
Gallardo, Carmina
Fernández, Paloma
Bello-Morales, Raquel
López-Guerrero, José Antonio
Chitko-Mckown, Carol G.
Richt, Jürgen A.
Revilla Novella, Yolanda
Kansas State University
Ministerio de Ciencia e Innovación (España)
African swine fever virus (ASFV) is a highly pathogenic, double-stranded DNA virus with a marked tropism for cells of the monocyte-macrophage lineage, affecting swine species and provoking severe economic losses and health threats. In the present study, four established porcine cell lines, IPAM-WT, IPAM-CD163, Câ2+ and WSL, were compared to porcine alveolar macrophage (PAM) in terms of surface marker phenotype, susceptibility to ASFV infection and virus production. The virulent ASFV Armenia/07, E70 or the naturally attenuated NHV/P68 strains were used as viral models. Cells expressed only low levels of specific receptors linked to the monocyte/macrophage lineage, with low levels of infection overall, with the exception of WSL, which showed more efficient production of strain NHV/P68 but not of strains E70 and Armenia/07.
2018-06-14T10:45:47Z
2018-06-14T10:45:47Z
2017-08-04
2018-06-14T10:45:48Z
artículo
Scientific Reports 7 (2017)
http://hdl.handle.net/10261/166323
10.1038/s41598-017-09948-x
http://dx.doi.org/10.13039/100007829
http://dx.doi.org/10.13039/501100004837
28871180
eng
Publisher's version
Sí
https://creativecommons.org/licenses/by/4.0/
openAccess
Nature Publishing Group