2024-03-28T12:08:10Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1582962022-05-23T07:28:09Zcom_10261_122com_10261_6com_10261_74col_10261_375col_10261_327
2017-12-19T07:33:33Z
urn:hdl:10261/158296
Production of "super-males" of asparagus by anther culture and its detection with SSR-ESTs
Regalado, J. J.
Carmona Martín, Elisabeth
Madrid, Eva
Carmona Martín, Elisabeth
Moreno Rojas, Rafael
Gil, Juan
Encina, C.L.
Junta de Andalucía
Haploid
Micropropagation
Morado de Huétor
Organogenesis
Sex-linked marker
Somaclonal variation
Anther culture is used to develop asparagus “super-male” (di-haploids) in asparagus, which can be used to develop “all-male” varieties, by crossing them with suitable females; their progenies will be formed only by males which is advantageous for producers. This report describe a new anther culture protocol adapted to “Morado de Huétor”, a Spanish tetraploid landrace, and studied the different factors involved in callus proliferation success from anther explants such as the microspore development stage, or the type of stress used to induce the symmetric division of the microspores, to obtain a high success rate (90 %). For plantlets regenerates from anther culture (PRACs) regeneration we develop a proliferation media supplemented with a combination of pCPA and BA able to induce callus proliferation and plantlet regeneration in the same step in a 50 % of calli, simplifying the procedure. The high percentage of heterozygous male recovery, originated from somatic cells, is an important problem in the anther culture, and to elucidate the origin of PRACs we have combined different tools: ploidy analysis, characterization with the linked sex-marker Asp1-T7 and with EST-SRRs. We can establish that 50 % of PRACs obtained in this work were regenerated from diploid microspores of “Morado de Huétor”, regenerating diploid, di-diploid and tetra-diploid plantlets. The di-diploids males (MMmm) would generate a ratio male:female of 5:1 (83.3 %) and the tetra-diploid males (MMMMmmmm) a ratio male:female of 69:1 (98.6 %), so the tetra-diploid males could be considered “super-males” and be used to develop “all-male” varieties of “Morado de Huétor”.
2017-12-19T07:33:33Z
2017-12-19T07:33:33Z
2016-01
artículo
Plant Cell, Tissue and Organ Culture 124(1): 119-135 (2016)
0167-6857
http://hdl.handle.net/10261/158296
10.1007/s11240-015-0880-6
1573-5044
http://dx.doi.org/10.13039/501100011011
eng
http://doi.org/10.1007/s11240-015-0880-6
Sí
closedAccess
Springer