2024-03-28T10:52:06Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1578522018-11-03T01:56:20Zcom_10261_74com_10261_6col_10261_327
2017-11-29T11:55:24Z
urn:hdl:10261/157852
Fate of Trichoderma harzianum in the olive rhizosphere: time course of the root colonization process and interaction with the fungal pathogen Verticillium dahliae
Ruano Rosa, David
Prieto, Pilar
Rincón Romero, Ana María
Gómez-Rodríguez, María Victoria
Valderrama, Raquel
Barroso-Albarracín, Juan Bautista
Mercado-Blanco, Jesús
European Commission
Ministerio de Economía y Competitividad (España)
Junta de Andalucía
Chlamydospores
Confocal laser scanning microscopy
Mycoparasitism
Olea europaea L.
TrichoRoot colonizationderma harzianum Rifai
Verticillium dahliae Kleb.
Verticillium wilt
Trichoderma harzianum Rifai is a well-known biological control agent (BCA) effective against a wide range of phytopathogens. Since colonization and persistence in the target niche is crucial for biocontrol effectiveness we aimed to: (i) shed light on the olive roots colonization process by T. harzianum CECT 2413, (ii) unravel the fate of its biomass upon application, and (iii) study the in planta interaction with the soil-borne pathogen Verticillium dahliae Kleb. Fluorescently-tagged derivatives of CECT 2413 and V. dahliae and confocal laser scanning microscopy were used. In vitro assays showed for the first time mycoparasitism of V. dahliae by T. harzianum, evidenced by events such as hyphal coiling. In planta assays revealed that CECT 2413 profusely colonized the rhizoplane of olive roots. Interestingly, biomass of the BCA was visualized mainly as chlamydospores. This observation was independent on the presence or absence of the pathogen. Evidence of inner colonization of olive roots by CECT 2413 was not obtained. These results suggest that CECT 2413 is not able to persist in a metabolically-active form when applied as a spore suspension. This may have strong implications in the way this BCA should be introduced and/or formulated to be effective against Verticillium wilt of olive.
2017-11-29T11:55:24Z
2017-11-29T11:55:24Z
2016-06
artículo
BioControl 61(3): 269-282 (2016)
1386-6141
http://hdl.handle.net/10261/157852
10.1007/s10526-015-9706-z
1573-8248
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100011011
eng
Postprint
http://doi.org/10.1007/s10526-015-9706-z
Sí
openAccess
Springer