2024-03-29T02:01:43Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1528482021-12-27T16:45:51Zcom_10261_34com_10261_5com_10261_44755com_10261_1col_10261_287col_10261_44757
2017-07-14T11:06:49Z
urn:hdl:10261/152848
The two kinases, AbrC1 and AbrC2, of the atypical two-component system AbrC are needed to regulate antibiotic production and differentiation in Streptomyces coelicolor
Rodríguez, Héctor
Rico, Sergio
Yepes, Ana
Franco-Echevarría, E.
Antoraz, Sergio
Santamaría, Ramón I.
Díaz, Margarita
Ministerio de Ciencia e Innovación (España)
Consejo Superior de Investigaciones Científicas (España)
Junta de Castilla y León
Fundación Botín
Two-component systems (TCSs) are the most important sensing mechanisms in bacteria. In Streptomyces, TCSs-mediated responses to environmental stimuli are involved in the regulation of antibiotic production. This study examines the individual role of two histidine kinases (HKs), AbrC1 and AbrC2, which form part of an atypical TCS in Streptomyces coelicolor. qRT-PCR analysis of the expression of both kinases demonstrated that both are expressed at similar levels in NB and NMMP media. Single deletion of abrC1 elicited a significant increase in antibiotic production, while deletion of abrC2 did not have any clear effect. The origin of this phenotype, probably related to the differential phosphorylation ability of the two kinases, was also explored indirectly, analyzing the toxic phenotypes associated with high levels of phosphorylated RR. The higher the AbrC3 regulator phosphorylation rate, the greater the cell toxicity. For the first time, the present work shows in Streptomyces the combined involvement of two different HKs in the response of a regulator to environmental signals. Regarding the possible applications of this research, the fact that an abrC1 deletion mutant overproduces three of the S. coelicolor antibiotics makes this strain an excellent candidate as a host for the heterologous production of secondary metabolites.
2017-07-14T11:06:49Z
2017-07-14T11:06:49Z
2015-05-12
2017-07-14T11:06:49Z
artículo
Frontiers in Microbiology 6: 450 (2015)
1664-302X
http://hdl.handle.net/10261/152848
10.3389/fmicb.2015.00450
http://dx.doi.org/10.13039/501100003339
http://dx.doi.org/10.13039/501100006373
http://dx.doi.org/10.13039/501100004837
http://dx.doi.org/10.13039/501100014180
26029189
en
Publisher's version
http://dx.doi.org/10.3389/fmicb.2015.00450
Sí
http://creativecommons.org/licenses/by/4.0/
openAccess
Frontiers Media