2024-03-28T20:00:01Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1442782022-11-23T13:09:48Zcom_10261_108com_10261_8col_10261_361
2017-02-20T10:25:44Z
urn:hdl:10261/144278
Mapping protective epitopes in the tick and mosquito subolesin ortholog proteins
Prudencio, Carlos Roberto
Pérez de Lastra, José Manuel
Canales, Mario
Villar, Margarita
Fuente, José de la
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil)
Wellcome Trust
European Commission
Consejo Superior de Investigaciones Científicas (España)
CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)
Ministério da Educação (Brasil)
Tick
Vaccine
Akirin
Subolesin
Mosquito
Immunology
Ixodes
Aedes
The tick protective antigen, subolesin, is a structural and functional ortholog of insect akirins, an evolutionary conserved group of proteins that regulate gene expression thus affecting multiple cellular processes such as digestion, immune response, reproduction and development. In this study, we searched for common protective epitopes in tick and mosquito subolesin ortolog proteins. By combining the results of peptide and phage-display libraries scan analysis with sera from protected animals with computational modeling, three different epitope types (i) linear B-cell epitopes, (ii) conformational epitopes, and (iii) conformational discontinuous epitopes were identified. The determination of conserved protective epitopes in subolesin ortologs may lead to the development of a multi-target universal vaccine directed at the control of both arthropod infestations and reduction of vector capacity to transmit pathogens.
2017-02-20T10:25:44Z
2017-02-20T10:25:44Z
2010
2017-02-20T10:25:44Z
artículo
Vaccine 28(33): 5398-5406 (2010)
http://hdl.handle.net/10261/144278
10.1016/j.vaccine.2010.06.021
http://dx.doi.org/10.13039/501100002322
http://dx.doi.org/10.13039/100004440
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100003339
http://dx.doi.org/10.13039/100007652
http://dx.doi.org/10.13039/501100006366
eng
Sí
closedAccess
Elsevier