2024-03-29T11:14:20Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1380522021-12-27T16:36:43Zcom_10261_34com_10261_5com_10261_86com_10261_1col_10261_287col_10261_339
2016-10-14T11:12:42Z
urn:hdl:10261/138052
Refactoring the λ phage lytic/lysogenic decision with a synthetic regulator
Durante-Rodríguez, Gonzalo
Mancheño, Jose M.
Díaz, Eduardo
Carmona, Manuel
Ministerio de Economía y Competitividad (España)
European Commission
Azoarcus
Protein engineering
Synthetic biology
Transcriptional regulation
7 p.-5 fig.-1 tab.
In this work, we explore the refactoring of the circuitry of λ phage by engineering
a new-to-nature regulator that responds to an ad hoc input signal that behaves orthogonal with respect to the host cell. We tailored a chimeric regulator, termed Qλ, between the CI protein of the λ phage and the BzdR repressor from Azoarcus sp. strain CIB that responds to benzoyl-CoA. When the Qλ was expressed in the appropriate Escherichia coli cells, it was able to reprogram the lytic/lysogenic λ phage decision according to the intracellular production of benzoyl-CoA.Our results are also an example of how generating new artificial regulators that respond to effectors of choice may be useful to control different cellular processes.
2016-10-14T11:12:42Z
2016-10-14T11:12:42Z
2016-08
artículo
MicrobiologyOpen 5(4): 575–581 (2016)
http://hdl.handle.net/10261/138052
10.1002/mbo3.352
2045-8827
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/501100000780
26987659
eng
Publisher's version
http://dx.doi.org/ 10.1002/mbo3.352
Sí
info:eu-repo/grantAgreement/EC/FP7/ 311815
http://creativecommons.org/licenses/by/4.0/
openAccess
John Wiley & Sons