2024-03-28T19:01:57Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1342042018-09-05T07:53:58Zcom_10261_112com_10261_1col_10261_365
2016-06-29T09:35:47Z
urn:hdl:10261/134204
Simultaneous analysis of the expression of 14 genes with individual prognostic value in myelodysplastic syndrome patients at diagnosis: WT1 detection in peripheral blood adversely affects survival
Santamaría, Carlos
Cañizo, María Consuelo del
Díez-Campelo, María
Chillón, M. del Carmen
García-Sanz, Ramón
San Miguel, Jesús F.
González, Marcos
Celgene
Instituto de Salud Carlos III
Junta de Castilla y León
CSIC-USAL - Instituto de Biología Molecular y Celular del Cancer de Salamanca (IBMCC)
Several studies have evaluated the prognostic value of the individual expression of certain genes in patients with myelodysplastic syndromes (MDS). However, none of them includes their simultaneous analysis by quantitative polymerase chain reaction (PCR). We evaluated relative expression levels of 14 molecular markers in 193 peripheral blood samples from untreated MDS patients using real-time PCR. Detectable WT1 expression levels, low TET2, and low IER3 gene expression were the only markers showing in univariate analysis a poor prognostic value for all treatment-free (TFS), progression-free (PFS), and overall survival (OS). In multivariate analysis, molecular parameters associated with a shorter TFS were: WT1 detection (p=0.014), low TET2 (p=0.002), and low IER3 expression (p=0.025). WT1 detection (p=0.006) and low TET2 (p=0.006) expression were associated with a shorter PFS when multivariate analysis was carried out by including only molecular markers. Molecular values with an independent value in OS were: WT1 detection (p=0.003), high EVI1 expression (p=0.001), and undetectatable p15-CDKN2B (p=0.037). WT1 expressers were associated with adverse clinical-biological features, high IPSS and WPSS scoring, and unfavorable molecular expression profile. In summary, detectable WT1 expression levels, and low TET2 and low IER3 expression in peripheral blood showed a strong association with adverse prognosis in MDS patients at diagnosis. However, WT1 was the only molecular marker displaying an independent prognostic value in both OS and TFS.
2016-06-29T09:35:47Z
2016-06-29T09:35:47Z
2012
2016-06-29T09:35:48Z
artículo
Annals of Hematology 91(12): 1887-1895 (2012)
http://hdl.handle.net/10261/134204
10.1007/s00277-012-1538-7
http://dx.doi.org/10.13039/100006436
http://dx.doi.org/10.13039/501100004587
http://dx.doi.org/10.13039/501100014180
eng
Sí
closedAccess
Springer