2024-03-28T23:33:33Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1338242020-08-17T12:00:39Zcom_10261_81com_10261_5col_10261_334
2016-06-21T10:17:03Z
urn:hdl:10261/133824
X-ray analysis of gastrointestinal motility in conscious mice. Effects of morphine and comparison with rats
Girón, Rocío
Pérez-García, I.
Abalo, Raquel
Ministerio de Educación y Ciencia (España)
Comunidad de Madrid
Rat
Morphine
Gastrointestinal motility
Mouse
Radiology
Barium marker
Background: Non-invasive methods to study gastrointestinal (GI) motility are of high interest, particularly in chronic studies. Amongst these, radiographic techniques after contrast intragastric administration may offer many advantages. In previous studies, we have successfully and reproducibly applied these techniques together with a semiquantitative analysis method to characterize the effect of different drugs, acutely or repeatedly administered in rat models, but we have never before used these techniques in mice. These are very convenient in basic research. Our aim was to determine if our method is also valid in mice. Additionally, we determined the effect of morphine on GI motor function in both species. Methods: Animals received an intraperitoneal administration of morphine (at 10 and 5 mg/kg for rats and mice, respectively). Twenty min later, barium contrast (at 2 g/mL) was gavaged (2.5 and 0.4 mL for rats and mice respectively) and serial X-rays were obtained 0-8 h after contrast. X-rays were analyzed as previously described, using a semiquantitative score to build motility curves for each GI region. Key Results: Motility was much faster in mice than in rats for all GI regions. Morphine at the doses used significantly depressed motility in both species to a similar extent if the whole gut or the upper GI regions (stomach, small intestine) were considered, although its effect seemed to be more intense in the lower GI regions (caecum, colorectum) in rats than in mice. Conclusions & Inferences: We have validated our X-rays method for its use in mice.
2016-06-21T10:17:03Z
2016-06-21T10:17:03Z
2016
2016-06-21T10:17:04Z
artículo
Neurogastroenterology and Motility 28: 74-84 (2016)
http://hdl.handle.net/10261/133824
10.1111/nmo.12699
http://dx.doi.org/10.13039/100012818
eng
http://dx.doi.org/10.1111/nmo.12699
Sí
closedAccess
John Wiley & Sons