2024-03-28T16:04:41Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1315102021-12-27T16:07:20Zcom_10261_10252com_10261_3col_10261_10253
2016-04-28T08:52:24Z
urn:hdl:10261/131510
Direct detection of protein biomarkers in human fluids using site-specific antibody immobilization strategies
Soler, María
Estévez, M. Carmen
Álvarez, Mar
Otte, Marinus A.
Sepúlveda, Borja
Lechuga, Laura M.
Consejo Superior de Investigaciones Científicas (España)
Ministerio de Economía y Competitividad (España)
Clinical diagnosis
Gold nanodisks
Nanoplasmonic biosensor
SPR biosensor
Direct immunoassay
ProLinker™
Antibody immobilization
This is an open access article distributed under the Creative Commons Attribution License (CC BY).
This article belongs to the Special Issue Plasmonics and Nanoplasmonics Biosensors.
Design of an optimal surface biofunctionalization still remains an important challenge for the application of biosensors in clinical practice and therapeutic follow-up. Optical biosensors offer real-time monitoring and highly sensitive label-free analysis, along with great potential to be transferred to portable devices. When applied in direct immunoassays, their analytical features depend strongly on the antibody immobilization strategy. A strategy for correct immobilization of antibodies based on the use of ProLinker™ has been evaluated and optimized in terms of sensitivity, selectivity, stability and reproducibility. Special effort has been focused on avoiding antibody manipulation, preventing nonspecific adsorption and obtaining a robust biosurface with regeneration capabilities. ProLinker™-based approach has demonstrated to fulfill those crucial requirements and, in combination with PEG-derivative compounds, has shown encouraging results for direct detection in biological fluids, such as pure urine or diluted serum. Furthermore, we have implemented the ProLinker™ strategy to a novel nanoplasmonic-based biosensor resulting in promising advantages for its application in clinical and biomedical diagnosis.
2016-04-28T08:52:24Z
2016-04-28T08:52:24Z
2014
2016-04-28T08:52:24Z
artículo
Sensors 14(2): 2239-2258 (2014)
http://hdl.handle.net/10261/131510
10.3390/s140202239
http://dx.doi.org/10.13039/501100003339
http://dx.doi.org/10.13039/501100003329
24481229
eng
Publisher's version
http://dx.doi.org/10.3390/s140202239
Sí
http://creativecommons.org/licenses/by/3.0/
openAccess
Multidisciplinary Digital Publishing Institute