2024-03-28T10:26:26Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1239932019-06-11T12:55:21Zcom_10261_77com_10261_8col_10261_330
2015-10-28T10:53:08Z
urn:hdl:10261/123993
Microsatellite DNA capture from enriched libraries
González, Elena G.
Zardoya, Rafael
Microsatellites are DNA sequences of tandem repeats of one to six nucleotides, which are highly polymorphic, and thus the molecular markers of choice in many kinship, population genetic, and conservation studies. There have been significant technical improvements since the early methods for microsatellite isolation were developed, and today the most common procedures take advantage of the hybrid capture methods of enriched-targeted microsatellite DNA. Furthermore, recent advents in sequencing technologies (i.e., next-generation sequencing, NGS) have fostered the mining of microsatellite markers in non-model organisms, affording a cost-effective way of obtaining a large amount of sequence data potentially useful for loci characterization. The rapid improvements of NGS platforms together with the increase in available microsatellite information open new avenues to the understanding of the evolutionary forces that shape genetic structuring in wild populations. Here, we provide detailed methodological procedures for microsatellite isolation based on the screening of GT microsatellite-enriched libraries, either by cloning and Sanger sequencing of positive clones or by direct NGS. Guides for designing new species-specific primers and basic genotyping are also given. © Springer Science+Business Media, LLC 2013.
2015-10-28T10:53:08Z
2015-10-28T10:53:08Z
2013-03-15
2015-10-28T10:53:08Z
artículo
Methods in Molecular Biology 1006: 67-87 (2013)
http://hdl.handle.net/10261/123993
10.1007/978-1-62703-389-3-5
eng
Sí
closedAccess
Humana Press