2024-03-28T07:56:44Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/109472019-11-14T10:01:58Zcom_10261_80com_10261_1col_10261_333
2009-02-24T13:09:07Z
urn:hdl:10261/10947
In vivo photosystem I reduction in thermophilic and mesophilic cyanobacteria: The thermal resistance of the process is limited by factors other than the unfolding of the partners
Durán, Raúl V.
Hervás, Manuel
Rosa, Miguel A. de la
Navarro, José A.
Cyanobacteria
Cytochrome c6
Laser flash spectroscopy
Nostoc
Photosynthesis
Phormidium
Plastocyanin
Synechocystis
Thermostability
6 pages, 3 figures, 2 tables.-- PMID: 15992773 [PubMed].-- Printed version published on Aug 19, 2005.
Photosystem I reduction by plastocyanin and cytochrome c6 in cyanobacteria has been extensively studied in vitro, but much less information is provided on this process inside the cell. Here, we report an analysis of the electron transfer from both plastocyanin and cytochrome c6 to photosystem I in intact cells of several cyanobacterial species, including a comparative study of the temperature effect in mesophilic and thermophilic organisms. Our data show that cytochrome c6 reduces photosystem I by following a reaction mechanism involving complex formation, whereas the copper–protein follows a simpler collisional mechanism. These results contrast with previous kinetic studies in vitro. The effect of temperature on photosystem I reduction leads us to conclude that the thermal resistance of this process is determined by factors other than the proper stability of the protein partners.
2009-02-24T13:09:07Z
2009-02-24T13:09:07Z
2005-06-24
artículo
Biochemical and Biophysical Research Communications 334(1): 170-175 (2005)
0006-291X
http://hdl.handle.net/10261/10947
10.1016/j.bbrc.2005.06.070
eng
http://dx.doi.org/10.1016/j.bbrc.2005.06.070
closedAccess
Elsevier