Work: Among-population variation and plasticity to drought of Atlantic, Mediterranean and interprovenance hybrid populations of Maritime pine [Dataset], 2014

Journal: Tree Genetics & Genomes (DOI:10.1007/s11295-014-0753-x)

Authors: de la Mata, Raúl; Merlo, Esther; Zas, Rafael

Issue date: 2014-05-28

Identifier: 

Description: This dataset compiles quantitative information about a greenhouse controlled experiment with two experimental treatments of water availability 
performed from August 10th, 2007 to February 5th, 2008 [180 days]. Atlantic, Mediterraenan and interprovenance hybrid populations between them of martitime pine (Pinus pinaster Ait.) were tested.
We assessed survival, growth and biomass allocation among different plant functional tissues as indicators of drought resistance. The explanation of dataset values are attached.

Methodology: Genetic material: The genetic material studied consisted of 12 Pinus pinaster populations, five of which were pure provenances and seven of which were interprovenance hybrids between them. 
The five pure populations included one of pure Atlantic origin (from the coastal area of Galicia, NW Spain), one from the Mediterranean region (from Montaña de Soria-Burgos, Central Spain), 
and three populations selected from an Atlantic area with a slight Mediterranean influence (from an inland area of Galicia). In addition to the five open pollinated populations, we obtained seven 
interprovenance hybrid populations by means of controlled pollinations. In Montaña de Soria-Burgos seed orchard, three genotypes were pollinated with pollen mixtures 
from the selected genotypes of the four Atlantic populations. In the Coastal-Galicia seed orchard, three selected genotypes were pollinated with pollen mixtures from the three inland 
Galicia populations (but only pollinations with IN1 were successful) and also from two extra French provenances: Landes (L), and Corsica. Because available seeds were insufficient to meet all our needs 
for plant material, we clonally replicated each individual by vegetative propagation using mini-cuttings. Experimental design, treatments and assessed variables: The study was conducted in a greenhouse under natural light and temperatures ranging from 12 to 30 ºC. The final containers were individual 650 cm3 pots 
situated on trays with 6 × 3 pots per tray. Each pot was filled with 840 g of washed and sieved river granitic sand, mixed with 1 g of gradual release fertilizer. 
Plants were arranged following a split-plot design replicated in seven blocks, with two water availability treatments acting as the main factor (whole plots) and the 12 populations as 
the split factor (split-plots), and a total of 432 plants. Half of the plants were subjected to a stress-free water regime, while the other half suffered a water deficit intensified over time.
In the well-watered treatment the substrate was kept at a mean saturation level of 80-90% throughout the whole experimental time, while in the drought-stressed treatment the substrate was kept at 
30-40% of the mean saturation level for the first two months and then reduced to 20-30% for the last four months.
Plant height was measured before starting the treatment applications and six months after (February 5th, 2008), computing thereafter height growth increment (ΔH, cm) for both stressed and non-stressed plants. Survival was 
also recorded by means of a binary variable (SUR) at the end of the experiment (February 5th 2008).
The harvested plants were then partitioned into five fractions based on the different functional properties of tissues, and then oven-dried (80° C to constant biomass) for biomass determination and weighed (± 0.001 g) to 
obtain the dry weights of stems (SDW), coarse roots (≥2 mm diameter, CRDW), fine roots (<2 mm diameter, FRDW), adult needles (ANDW) and juvenile needles (JNDW).

Access and reuse: This dataset is subject to a Creative Commons Reconocimiento-NoComercial-CompartirIgual 4.0 Internacional License.

Format: Excel

Additional information: Explanation of dataset values are attached to the dataset. Dataset analysed at 'de la Mata, R; Merlo, E and Zas, R.' (2014; DOI:10.1007/s11295-014-0753-x)

Sponsorship: This study was partially supported by the research grants RTA07-100, PSE310000 and AGL2010-18724-COMPROPIN (Spanish Government). Raúl de la Mata is undertaking a postdoctoral scholarship at 
The University of Montana funded by the Barrié Foundation